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KMID : 0894520090130040353
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Development & Reproduction
2009 Volume.13 No. 4 p.353 ~ p.362
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Effects of Gonadotropin Releasing Hormone on Steroidogenesis and Apoptosis of Human Granulosa-Lutein Cells
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Lee Hyo-Jin
Yang Hyun-Won
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Abstract
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GnRH and its receptor are known to express locally in the ovary and to regulate the ovarian function by affecting on granulosa and lutein cells. It has been reported that GnRH directly causes apoptosis in the granulosa and lutein cells of the ovary. However, whether the apoptosis of the cells by GnRH is recovered by FSH as an anti-apoptotic factor is not yet known. In this study, we evaluated the apoptosis and the production of progesterone (P₄) and estradiol (E₂) after treatment with 5, 50, and 100 ng/㎖ GnRH and 1 IU/ml FSH in the granulosa-lutein cells that are obtained during oocyte-retrieval for IVF-ET. Results of DNA fragment analysis and TUNEL assay demonstrated that DNA fragmentation and the rate of apoptotic cells were increased in a dose-dependent manner showing a significant increase in the cells treated with 100 ng/㎖ GnRH. In addition, we found that FSH suppresses the apoptosis of the cells induced by GnRH. In the results of chemiluminescence assay for P₄ and E₂, P₄ production was decreased by GnRH treatment, whereas E₂ production was not changed. We also demonstrated that FSH inhibits the suppressive effect of GnRH on P₄ production as the result of apoptosis. The present results suggest that GnRH agonist using in ovarian hyperstimulation protocol might induce the dysfunction of the ovary, but its function could be recovered by FSH. These results also will be expected to use as the basic data to elucidate the physiological role of GnRH and to develop new ovarian hyperstimulation protocols for IVF-ET.
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KEYWORD
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Apoptosis, FSH, GnRH, Granulosa-lutein cell, Ovarian hyperstimulation, IVF-ET
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